anti popdc3 antibody  (Proteintech)

Journal: Cell Death & Disease

Article Title: A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

doi: 10.1038/s41419-025-07410-9

Figure Lengend Snippet: POPDC3 immunohistochemistry (IHC) microarray of NSCLC tissues and their adjacent normal tissues were shown ( A , C ) and the representative IHC images of each microarray were presented ( B , D ). The expression of POPDC3 was quantified and compared in LUAD and LUSC tissues versus adjacent normal tissues, analyzed both as unpaired and paired samples ( E – H ). The association between POPDC3 intensity scores and OS events was investigated for both LUAD ( I ) and LUSC ( J ) cases. KM survival analyses based on POPDC3 expression in LUAD patients ( K ) and LUSC patients ( L ) were shown. * P < 0.05; ** P < 0.01; *** P < 0.001; “n.s.” stands for P > 0.05. Scale bar = 100 μm.

Article Snippet: For IHC analysis, tissue sections underwent antigen retrieval and blocking, followed by overnight incubation with anti-POPDC3 antibody (ProteinTech) at 4 °C.

Techniques: Immunohistochemistry, Microarray, Expressing

Journal: Cell Death & Disease

Article Title: A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

doi: 10.1038/s41419-025-07410-9

Figure Lengend Snippet: Established NSCLC cell lines (A549, H1299) were genetically modified using shRNA sequences targeting POPDC3 (“sh-POPDC3 -S1/S2”) or a non-targeting scramble control shRNA (“shc”). Cells were then cultivated and screened for changes in the expression of listed genes and proteins ( A – E ); The impact of POPDC3 suppression on cell behaviors was assessed over indicated cultivation durations, examining cell viability ( F ), colony-forming capabilities ( G ), EdU incorporation ( H ), and cell motility ( I ). The cell migration ( J ) and invasion ( K ) were evaluated with established assays. The expression of listed proteins was analyzed using Western blotting ( L ). Error bars stand for mean ± standard deviation (SD, n = 3). Statistical significance is denoted as * P < 0.05; ** P < 0.01; *** P < 0.001 versus “shc” cells, while “n.s.” stands for P > 0.05. Scale bar = 100 μm.

Article Snippet: For IHC analysis, tissue sections underwent antigen retrieval and blocking, followed by overnight incubation with anti-POPDC3 antibody (ProteinTech) at 4 °C.

Techniques: Genetically Modified, shRNA, Control, Expressing, Migration, Western Blot, Standard Deviation

Journal: Cell Death & Disease

Article Title: A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

doi: 10.1038/s41419-025-07410-9

Figure Lengend Snippet: Established NSCLC cell lines (A549, H1299) ( A – I ) along with primary human NSCLC cells (priNSCLC-1) ( J – O ) were genetically modified to overexpress POPDC3 via a lentiviral vector (“POPDC3-OE”) compared to a control vector (“Vec”), employing puromycin selection. The expression of listed genes and proteins was shown ( A – D , J – L ). Subsequently, after a designated incubation period, the cells underwent various assays to assess cell viability ( E ), cell colony formation ( F , M ), cell proliferation indicated by EdU incorporation ( G , N ), as well as migration ( H ) and invasion ( I , O ). Error bars stand for mean ± standard deviation (SD, n = 3). Statistical significance is denoted as * P < 0.05; ** P < 0.01; *** P < 0.001 versus “Vec” cells, while “n.s.” stands for P > 0.05. Scale bar = 100 μm.

Article Snippet: For IHC analysis, tissue sections underwent antigen retrieval and blocking, followed by overnight incubation with anti-POPDC3 antibody (ProteinTech) at 4 °C.

Techniques: Genetically Modified, Plasmid Preparation, Control, Selection, Expressing, Incubation, Migration, Standard Deviation

Journal: Cell Death & Disease

Article Title: A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

doi: 10.1038/s41419-025-07410-9

Figure Lengend Snippet: Female BALB/c nude mice were implanted with H1299 cells expressing POPDC3-targeting shRNA (“POPDC3-sh-S1”, “POPDC3-sh-S2”) or a non-targeting scramble control shRNA (“shC”) to establish the experimental model. Tumor growth was monitored by measuring tumor volumes ( A ) and recording mouse body weights ( F ) at 5-day intervals. After 30 days, tumors were surgically excised ( B , C ), and their weights were measured ( D ). A tumor-free survival curve was also displayed ( E ). Within the harvested tumor tissues, the levels of listed genes ( G ) were evaluated, with results quantified. Representative IHC images of POPDC3 ( H ) and Ki67 ( I ) in tumor tissues were shown. Additionally, representative images of TUNEL staining on tumor tissues were presented ( J ). The expression of apoptosis-related proteins was analyzed using Western blotting ( K ). Statistical significance is denoted as * P < 0.05; ** P < 0.01; *** P < 0.001 versus “shc” cells, while “n.s.” stands for P > 0.05. Scale bar = 50 μm.

Article Snippet: For IHC analysis, tissue sections underwent antigen retrieval and blocking, followed by overnight incubation with anti-POPDC3 antibody (ProteinTech) at 4 °C.

Techniques: Expressing, shRNA, Control, TUNEL Assay, Staining, Western Blot

Journal: Cell Death & Disease

Article Title: A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

doi: 10.1038/s41419-025-07410-9

Figure Lengend Snippet: At 3 × 10 6 cells per mouse, priNSCLC-1 primary NSCLC cells with the lentiviral POPDC3 overexpression construct (“POPDC3-OE”) or the corresponding vector (“Vec”) were s.c. injected to the nude mice to establish the experimental model. Tumor growth was monitored by measuring tumor volumes ( A ) and recording mouse body weights ( C ) at 5-day intervals. After 30 days, tumors were surgically excised, and their weights were measured ( B ). Within the harvested tumor tissues, the levels of listed genes and proteins ( D–H ) were evaluated, with results quantified. Representative IHC images of POPDC3 in tumor tissues were shown ( I ). Data were presented as mean ± standard deviation (SD). Statistical significance is denoted as * P < 0.05; ** P < 0.01; *** P < 0.001 versus “Vec” cells, while “n.s.” stands for P > 0.05. Scale bar = 50 μm.

Article Snippet: For IHC analysis, tissue sections underwent antigen retrieval and blocking, followed by overnight incubation with anti-POPDC3 antibody (ProteinTech) at 4 °C.

Techniques: Over Expression, Construct, Plasmid Preparation, Injection, Standard Deviation

Journal: Cell Death & Disease

Article Title: A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

doi: 10.1038/s41419-025-07410-9

Figure Lengend Snippet: Representative mIHC images of patient samples with high-POPDC3 expression ( A ) and low-POPDC3 expression ( B ) were presented, with each color panel shown alongside. Various markers were visualized by distinct colors, including POPDC3 in red, CD8 in turquoise, CD4 in green, Pan-CK in pink and PD1 in gold, with DAPI used for counterstaining. Quantitative comparison of POPDC3 distribution within NSCLC tissue relative to normal tissue was conducted using an H-score ( C ). The association between POPDC3 expression levels and the presence of CD8 + ( D ) and CD4 + T cells ( E ) was investigated. The proportion of PD1 + cells ( F ) and CD4 + PD1 + cells ( G ) was explored by comparing the high versus low-POPDC3-expressing groups within the tumor tissues ( F ). At 3 × 10 6 cells per mouse, Lewis lung carcinoma (LLC) cells with the lentiviral POPDC3 overexpression construct (“POPDC3-OE”) or the corresponding vector (“Vec”) were s.c . injected to the C57BL/6 J mice to establish the experimental model. After 30 days, the mice were sacrificed, and the NSCLC tumor tissues were fixed with 10% formaldehyde, sectioned and analyzed by immunohistochemical staining ( G ). Representative IHC staining of POPDC3, CD4 and PD1, and their quantitative analyses in LLC xenograft tissues in C57BL/6J mice ( H ). * P < 0.05; ** P < 0.01; *** P < 0.001; “n.s.” stands for P > 0.05. Scale bar = 50 μm.

Article Snippet: For IHC analysis, tissue sections underwent antigen retrieval and blocking, followed by overnight incubation with anti-POPDC3 antibody (ProteinTech) at 4 °C.

Techniques: Expressing, Comparison, Over Expression, Construct, Plasmid Preparation, Injection, Immunohistochemical staining, Staining, Immunohistochemistry